Sequences with good randomness properties are quite important for stream ciphers. In this paper, a new class of quaternary sequences is constructed by using generalized cyclotomic classes of $\mathbb{Z}_{2p^m}$ $(m\geq1)$. The exact values of autocorrelation of these sequences are determined based on cyclotomic numbers of order $2$ with respect to $p^m$. Results show that the presented sequences have the autocorrelations with at most $4$ values.