This study analyzes the performance of positioning techniques based on configuration changes of 5G New Radio signals. In 5G networks, a terminal position is determined from the Time of Arrival of Positioning Reference Signals transmitted by base stations. We propose an algorithm that improves TOA accuracy under low sampling rate constraints and implement 5G PRS for positioning in a software defined modem. We also examine how flexible time frequency resource allocation of PRS affects TOA estimation accuracy and discuss optimal PRS configurations for a given signal environment.