We present a new correspondence between acyclic orientations and coloring of a signed graph (symmetric graph). Goodall et al. introduced a bivariate chromatic polynomial $χ_G(k,l)$ that counts the number of signed colorings using colors $0,\pm1,\dots,\pm k$ along with $l-1$ symmetric colors $0_1,\dots,0_{l-1}$. We show that the evaluation of the bivariate chromatic polynomial $|χ_G(-1,2)|$ is equal to the number of acyclic orientations of the signed graph modulo the equivalence relation generated by swapping sources and sinks. We present three proofs of this fact, a proof using toric hyperplane arrangements, a proof using deletion-contraction, and a direct proof.