Cumulative effect in social contagions underlies many studies on the spread of innovation, behaviors, and influence. However, few large-scale empirical studies are conducted to validate the existence of cumulative effect in the information diffusion on social networks. In this paper, using the population-scale dataset from the largest Chinese microblogging website, we conduct a comprehensive study on the cumulative effect in information diffusion. We base our study on the diffusion network of each message, where nodes are the involved users and links are the following relationships among them. We find that multiple exposures to the same message indeed increase the possibility of forwarding it. However, additional exposures cannot further improve the chance of forwarding when the number of exposures crosses its peak at two. This finding questions the cumulative effect hypothesis in information diffusion. Furthermore, to clarify the forwarding preference among users, we investigate both the structural motif of the diffusion network and the temporal pattern of information diffusion process among users. The patterns provide vital insight for understanding the variation of message popularity and explain the characteristics of diffusion networks.