Centrality measures are used in network science to identify the most important vertices for transmission of information and dynamics on a graph. One of these measures, introduced by Estrada and collaborators, is the $β$-subgraph centrality, which is based on the exponential of the matrix $βA$, where $A$ is the adjacency matrix of the graph and $β$ is a real parameter ("inverse temperature"). We prove that for algebraic $β$, two vertices with equal $β$-subgraph centrality are necessarily cospectral. We further show that two such vertices must have the same degree and eigenvector centralities. Our results settle a conjecture of Estrada and a generalization of it due to Kloster, Král and Sullivan. We also discuss possible extensions of our results.