Over the years, data mining has attracted most of the attention from the research community. The researchers attempt to develop faster, more scalable algorithms to navigate over the ever increasing volumes of spatial gene expression data in search of meaningful patterns. Association rules are a data mining technique that tries to identify intrinsic patterns in spatial gene expression data. It has been widely used in different applications, a lot of algorithms introduced to discover these rules. However Priori like algorithms has been used to find positive association rules. In contrast to positive rules, negative rules encapsulate relationship between the occurrences of one set of items with absence of the other set of items. In this paper, an algorithm for mining negative association rules from spatial gene expression data is introduced. The algorithm intends to discover the negative association rules which are complementary to the association rules often generated by Priori like algorithm. Our study shows that negative association rules can be discovered efficiently from spatial gene expression data.