Precise localization and recognition of flowers are crucial for advancing automated agriculture, particularly in plant phenotyping, crop estimation, and yield monitoring. This paper benchmarks several YOLO architectures such as YOLOv5s, YOLOv8n/s/m, and YOLOv12n for flower object detection under two annotation regimes: single-image single-bounding box (SISBB) and single-image multiple-bounding box (SIMBB). The FloralSix dataset, comprising 2,816 high-resolution photos of six different flower species, is also introduced. It is annotated for both dense (clustered) and sparse (isolated) scenarios. The models were evaluated using Precision, Recall, and Mean Average Precision (mAP) at IoU thresholds of 0.5 (mAP@0.5) and 0.5-0.95 (mAP@0.5:0.95). In SISBB, YOLOv8m (SGD) achieved the best results with Precision 0.956, Recall 0.951, mAP@0.5 0.978, and mAP@0.5:0.95 0.865, illustrating strong accuracy in detecting isolated flowers. With mAP@0.5 0.934 and mAP@0.5:0.95 0.752, YOLOv12n (SGD) outperformed the more complicated SIMBB scenario, proving robustness in dense, multi-object detection. Results show how annotation density, IoU thresholds, and model size interact: recall-optimized models perform better in crowded environments, whereas precision-oriented models perform best in sparse scenarios. In both cases, the Stochastic Gradient Descent (SGD) optimizer consistently performed better than alternatives. These density-sensitive sensors are helpful for non-destructive crop analysis, growth tracking, robotic pollination, and stress evaluation.