Large Language Models (LLMs) are increasingly deployed across diverse domains but often exhibit disparities in how they handle real-life queries. To systematically investigate these effects within various disability contexts, we introduce \textbf{AccessEval (Accessibility Evaluation)}, a benchmark evaluating 21 closed- and open-source LLMs across 6 real-world domains and 9 disability types using paired Neutral and Disability-Aware Queries. We evaluated model outputs with metrics for sentiment, social perception, and factual accuracy. Our analysis reveals that responses to disability-aware queries tend to have a more negative tone, increased stereotyping, and higher factual error compared to neutral queries. These effects show notable variation by domain and disability type, with disabilities affecting hearing, speech, and mobility disproportionately impacted. These disparities reflect persistent forms of ableism embedded in model behavior. By examining model performance in real-world decision-making contexts, we better illuminate how such biases can translate into tangible harms for disabled users. This framing helps bridges the gap between technical evaluation and user impact, reinforcing importance of bias mitigation in day-to-day applications. Our dataset is publicly available at: https://huggingface.co/datasets/Srikant86/AccessEval