Serious games are simulation software designed to assist people in learning the practical concepts of various application fields such as Health, wellness, Education and Culture. People improve their individual knowledge, skills and attitude through training. This study identified the changing trends with existing studied applications, approaches and methods. We collected 37 papers from Google Scholar, Elsevier, Springer, IEEE Xplore and ACM Digital library. We have collected the evidence answer to six research questions and analyzed the result and identify the change in trends with the comparison with previous systematic literature review (SLR) results. We achieved the best results by techniques (questionnaires and interviews) and procedure (pre/post). Our findings will be useful for practitioners and researchers who can test serious games in different fields.