We introduce ScreenQA, a novel benchmarking dataset designed to advance screen content understanding through question answering. The existing screen datasets are focused either on low-level structural and component understanding, or on a much higher-level composite task such as navigation and task completion for autonomous agents. ScreenQA attempts to bridge this gap. By annotating 86k question-answer pairs over the RICO dataset, we aim to benchmark the screen reading comprehension capacity, thereby laying the foundation for vision-based automation over screenshots. Our annotations encompass full answers, short answer phrases, and corresponding UI contents with bounding boxes, enabling four subtasks to address various application scenarios. We evaluate the dataset's efficacy using both open-weight and proprietary models in zero-shot, fine-tuned, and transfer learning settings. We further demonstrate positive transfer to web applications, highlighting its potential beyond mobile applications.