Safety and reliability are the main issues for designing assistance wearable virtual environment of technical gesture in aerospace, or health application domains. That needs the integration in the same isomorphic engineering framework of human requirements, systems requirements and the rationale of their relation to the natural and artifactual environment.To explore coupling integration and design functional organization of support technical gesture systems, firstly ecological psychologyprovides usa heuristicconcept: the affordance. On the other hand mathematical theory of integrative physiology provides us scientific concepts: the stabilizing auto-association principle and functional interaction.After demonstrating the epistemological consistence of these concepts, we define an isomorphic framework to describe and model human systems integration dedicated to human in-the-loop system engineering.We present an experimental approach of safe design of assistance wearable virtual environment of gesture based in laboratory and parabolic flights. On the results, we discuss the relevance of our conceptual approach and the applications to future assistance of gesture wearable systems engineering.