The pathway is a biological term that refers to a series of interactions between molecules in a cell that causes a certain product or a change in the cell. Pathway analysis is a powerful method for gene expression analysis. Through pathway maps, the lists of genes that are differentially expressed across the given phenotypes are translated into various biological phenomena. Visualizing a pathway map manually is a common practice nowadays because of the limitations of existing solutions to draw complicated graphs (i.e. directed graphs, graphs with edge crossings, etc). This project provides a solution to draw pathway maps automatically based on spectral graph theory and topological sort. Various methods are taken to enhance pathway maps' readability. Significant reductions in the number of edge crossings and the sum of adjacent nodes are achieved.