Sampling-based methods such as ReCom are widely used to audit redistricting plans for fairness, with the balanced spanning tree distribution playing a central role since it favors compact, contiguous, and population-balanced districts. However, whether such samples are truly representative or exhibit hidden biases remains an open question. In this work, we introduce the notion of separation fairness, which asks whether adjacent geographic units are separated with at most a constant probability (bounded away from one) in sampled redistricting plans. Focusing on grid graphs and two-district partitions, we prove that a smooth variant of the balanced spanning tree distribution satisfies separation fairness. Our results also provide theoretical support for popular MCMC methods like ReCom, suggesting that they maintain fairness at a granular level in the sampling process. Along the way, we develop tools for analyzing loop-erased random walks and partitions that may be of independent interest.