MALDI-tof - Jinji
2022-08-08
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via Home on Jinji
- Fill out the MALDI excel form, put the name of the isolates (2 spot per isolate), then two spots for BTS (control).
- Apply samples on the plate, one sample has two consecutive spots.Also two spots BTS at the end.
- Add 1 uL 70% formic acid to each sample, but not BTS. Let the plate to be dry.
- Add 1 uL Matrix to each spot, including BTS. Let the plate to be dry.
- Open the MALDI-tof machine.
- Check if the excel IDs match the plate.
- Use BTS to adjust, check the machine is in good working condition.
- Start reading the samples and copy the result.
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