Personality analysis from online short videos has gained prominence due to its applications in personalized recommendation systems, sentiment analysis, and human-computer interaction. Traditional assessment methods, such as questionnaires based on the Big Five Personality Framework, are limited by self-report biases and are impractical for large-scale or real-time analysis. Leveraging the rich, multi-modal data present in short videos offers a promising alternative for more accurate personality inference. However, integrating these diverse and asynchronous modalities poses significant challenges, particularly in aligning time-varying data and ensuring models generalize well to new domains with limited labeled data. In this paper, we propose a novel multi-modal personality analysis framework that addresses these challenges by synchronizing and integrating features from multiple modalities and enhancing model generalization through domain adaptation. We introduce a timestamp-based modality alignment mechanism that synchronizes data based on spoken word timestamps, ensuring accurate correspondence across modalities and facilitating effective feature integration. To capture temporal dependencies and inter-modal interactions, we employ Bidirectional Long Short-Term Memory networks and self-attention mechanisms, allowing the model to focus on the most informative features for personality prediction. Furthermore, we develop a gradient-based domain adaptation method that transfers knowledge from multiple source domains to improve performance in target domains with scarce labeled data. Extensive experiments on real-world datasets demonstrate that our framework significantly outperforms existing methods in personality prediction tasks, highlighting its effectiveness in capturing complex behavioral cues and robustness in adapting to new domains.