Vision-Language Models (VLMs) have achieved remarkable success, yet their reliance on massive datasets and unintended memorization of training data raise significant data security risk. Membership Inference Attacks (MIAs) aim to assess these risks by determining whether a data sample was included in a model's training set. However, existing MIA methods against VLMs face critical bottlenecks: gray-box method relies on internal logits that are typically restricted in real-world Application Programming Interfaces (APIs), while black-box method depends on large-scale statistical distributions, which struggle in single-sample scenarios. To this end, we investigate MIAs from the perspective of cross-modal semantic alignment, and observe that member images exhibit significantly stronger image-caption alignment due to training memorization, whereas generated captions for non-members may deviate from the original visual content. Leveraging this insight, we propose a novel MIA framework designed for strict black-box and single-sample setting that quantifies such alignment within a joint embedding space, thereby bypassing these unrealistic assumptions. We conducted extensive experiments on three open-source and two closed-source VLMs. On the VL-MIA/Flicker dataset, our method achieves an AUC of 0.821 against LLaVA-1.5, significantly outperforming existing baselines. Furthermore, it remains robust under diverse image perturbations, highlighting its practicality.