Symmetry arguments are frequently used -- often implicitly -- in mathematical modeling of natural selection. Symmetry simplifies the analysis of models and reduces the number of distinct population states to be considered. Here, I introduce a formal definition of symmetry in mathematical models of natural selection. This definition applies to a broad class of models that satisfy a minimal set of assumptions, using a framework developed in previous works. In this framework, population structure is represented by a set of sites at which alleles can live, and transitions occur via replacement of some alleles by copies of others. A symmetry is defined as a permutation of sites that preserves probabilities of replacement and mutation. The symmetries of a given selection process form a group, which acts on population states in a way that preserves the Markov chain representing selection. Applying classical results on group actions, I formally characterize the use of symmetry to reduce the states of this Markov chain, and obtain bounds on the number of states in the reduced chain.