1<asp:DataGrid id="DataGrid1" runat="server">
2    <Columns>
3     <asp:TemplateColumn>
4      <ItemTemplate>
5       <%# this.DataGrid1.Items.Count + 1%>
6      </ItemTemplate>
7     </asp:TemplateColumn>
8    </Columns>
9</asp:DataGrid>

1<asp:DataGrid id="DataGrid1" runat="server" AllowPaging="True">
2    <Columns>
3     <asp:TemplateColumn>
4      <ItemTemplate>
5       <%# this.DataGrid1.CurrentPageIndex * this.DataGrid1.PageSize + Container.ItemIndex + 1%>
6      </ItemTemplate>
7     </asp:TemplateColumn>
8    </Columns>
9</asp:DataGrid>

 1private int rowscount = 0;
 2         protected int RowsCount
 3         {
 4              get{ return rowscount;}
 5              set{ this.rowscount = value; }
 6         }
 7     
 8         private void Page_Load(object sender, System.EventArgs e)
 9         {
10              // 在此处放置用户代码以初始化页面
11              if(!IsPostBack)
12                   this.BindData();
13         }
14         private void BindData()
15         {
16              SqlConnection cn = new SqlConnection("server=(local);database=NorthWind;uid=sa;pwd=");
17              string str=@"SELECT Employees.EmployeeID, Orders.EmployeeID
18                                 FROM Employees INNER JOIN
19                       Orders ON Employees.EmployeeID = Orders.EmployeeID ";
20              SqlDataAdapter sqlda = new SqlDataAdapter(str,cn);
21              DataSet ds = new DataSet();
22              sqlda.Fill(ds);
23              this.RowsCount = ds.Tables[0].Rows.Count;
24              this.DataGrid1.DataSource = ds;
25              this.DataGrid1.DataBind();
26}

1<asp:DataGrid id="DataGrid1" runat="server" AllowPaging="True">
2                            <Columns>
3                                   <asp:TemplateColumn>
4                                          <ItemTemplate>
5                                                 <%# RowsCount - DataGrid1.CurrentPageIndex * DataGrid1.PageSize - Container.ItemIndex %>
6                                          </ItemTemplate>
7                                   </asp:TemplateColumn>
8                            </Columns>
9                     </asp:DataGrid>