Soil water content (SWC) is a key state variable of the climate system but is often uncertain in water balance monitoring, especially in alpine environments. SWC measurements can be challenging in alpine environments due to the topography and rocky soils. In 2022, the US Geological Survey's Next Generation Water Observing System Program began research to evaluate water balance monitoring technologies, including cosmic-ray neutron sensors (CRNS). This work evaluated the uncertainty resulting from network-wide calibration of CRNS for SWC monitoring in an alpine watershed and investigated the stability of the calibration parameters across space and time, focusing on potential influence of biomass dynamics. Fifteen stations with moderated and unmoderated (bare) CRNS were deployed and made operational within the Roaring Fork Basin in west-central Colorado. The root mean squared error of the network-wide calibration using the moderated CRNS was 0.042 or 0.047 cm³ cm⁻³, depending on the calibration equation used. Relative SWC dynamics from CRNS were correlated with the in situ probes with a correlation coefficient of 0.91 or 0.87 (depending on calibration equation). We did not find significant relationships between the calibration parameters and stationary site-specific variables. However, the calibration parameters derived from in situ probe SWC dynamics varied over time and were correlated with biomass proxies of cumulative growing degree-day, cumulative growing season index, and bare neutron counts. Future use of the CRNS network can leverage the reliable relative SWC data from network-wide calibration for watershed modeling and continue to research sensitivity of bare neutron measurements to biomass dynamics.