In this paper, we propose adaptive channel-matched detection (ACMD) for C-band 64-Gbit/s intensity-modulation and direct-detection (IM/DD) optical on-off keying (OOK) system over a 100-km dispersion-uncompensated link. The proposed ACMD can adaptively compensate most of the link distortions based on channel and noise characteristics, which includes a polynomial nonlinear equalizer (PNLE), a decision feedback equalizer (DFE) and maximum likelihood sequence estimation (MLSE). Based on the channel characteristics, PNLE eliminates the linear and nonlinear distortions, while the followed DFE compensates the spectral nulls caused by chromatic dispersion. Finally, based on the noise characteristics, a post filter can whiten the noise for implementing optimal signal detection using MLSE. To the best of our knowledge, we present a record C-band 64-Gbit/s IM/DD optical OOK system over a 100 km dispersion-uncompensated link achieving 7\% hard-decision forward error correction limit using only the proposed ACMD at the receiver side. In conclusion, ACMD-based C-band 64-Gbit/s optical OOK system shows great potential for future optical interconnects.