This paper investigates multi-target position estimation in cell-free massive multiple-input multiple-output (CF mMIMO) architectures, where orthogonal time frequency and space (OTFS) is used as an integrated sensing and communication (ISAC) signal. Closed-form expressions for the Cramér-Rao lower bound and the positioning error bound (PEB) in multi-target position estimation are derived, providing quantitative evaluations of sensing performance. To enhance the overall performance of the ISAC system, a power allocation algorithm is developed to maximize the minimum user communication signal-to-interference-plus-noise ratio while ensuring a specified sensing PEB requirement. The results validate the proposed PEB expression and its approximation, clearly illustrating the coordination gain enabled by ISAC. Further, the superiority of using the multi-static CF mMIMO architecture over traditional cellular ISAC is demonstrated, and the advantages of OTFS signals in high-mobility scenarios are highlighted.