The integration of sensing and communication enables wireless communication systems to serve environment-aware applications. In this paper, we propose to leverage sensing to enhance physical layer security (PLS) in multiuser communication systems in the presence of a suspicious target. To this end, we develop a two-phase framework to first estimate the location of the potential eavesdropper by sensing and then utilize the estimated information to enhance PLS for communication. In particular, in the first phase, a dual-functional radar and communication (DFRC) base station (BS) exploits a sensing signal to mitigate the sensing information uncertainty of the potential eavesdropper. Then, in the second phase, to facilitate joint sensing and secure communication, the DFRC BS employs beamforming and artificial noise to enhance secure communication. The design objective is to maximize the system sum rate while alleviating the information leakage by jointly optimizing the time allocation and beamforming policy. Capitalizing on monotonic optimization theory, we develop a two-layer globally optimal algorithm to reveal the performance upper bound of the considered system. Simulation results show that the proposed scheme achieves a significant sum rate gain over two baseline schemes that adopt existing techniques. Moreover, our results unveil that ISAC is a promising paradigm for enhancing secure communication in wireless networks.