Physical-layer key generation (PKG) based on channel reciprocity has recently emerged as a new technique to establish secret keys between devices. Most works focus on pairwise communication scenarios with single or small-scale antennas. However, the fifth generation (5G) wireless communications employ massive multiple-input multiple-output (MIMO) to support multiple users simultaneously, bringing serious overhead of reciprocal channel acquisition. This paper presents a multi-user secret key generation in massive MIMO wireless networks. We provide a beam domain channel model, in which different elements represent the channel gains from different transmit directions to different receive directions. Based on this channel model, we analyze the secret key rate and derive a closed-form expression under independent channel conditions. To maximize the sum secret key rate, we provide the optimal conditions for the Kronecker product of the precoding and receiving matrices and propose an algorithm to generate these matrices with pilot reuse. The proposed optimization design can significantly reduce the pilot overhead of the reciprocal channel state information acquisition. Furthermore, we analyze the security under the channel correlation between user terminals (UTs), and propose a low overhead multi-user secret key generation with non-overlapping beams between UTs. Simulation results demonstrate the near optimal performance of the proposed precoding and receiving matrices design and the advantages of the non-overlapping beam allocation.