We present a novel framework for line-of-sight (LoS) delay-Doppler (DD) estimation in dense scattering propagation environments. We present two time-frequency (TF) domain pilot sequences inspired by the Zadoff-Chu sequence that exhibit desirable autocorrelation properties. Further, we present a twisted convolution-based approach for LoS DD estimation directly from the TF-domain received signal, avoiding an additional TF to DD transformation, which is commonly found in literature. Numerical results from simulations demonstrate that the proposed framework significantly outperforms traditional single-carrier Zadoff-Chu sequences in both delay and Doppler estimation over a wide range of Rician fading factor and SNR values.