Flow 2.0 is an end-to-end easy-of-use software that allows us to quickly, robustly and accurately perform a batch process real-time phase contrast data and multivariate analysis of the effect of respiration on cerebral fluids circulation. Synopsis (99/100) Real-time phase contrast sequences (RT-PC) have potential value as a scientific and clinical tool in quantifying the effects of respiration on cerebral circulation. To simplify its complicated post-processing process, we developed Flow 2.0 software, which provides a complete post-processing workflow including converting DICOM data, image segmentation, image processing, data extraction, background field correction, antialiasing filter, signal processing and analysis and a novel time-domain method for quantifying the effect of respiration on the cerebral circulation. This end-to-end software allows us to quickly, robustly and accurately perform batch process RT-PC and multivariate analysis of the effects of respiration on cerebral circulation.