This paper develops a new genetic algorithm based resource allocation (GA-RA) technique for energy-efficient throughout maximization in multi-user massive multiple-input multiple-output (MU-mMIMO) systems using orthogonal frequency division multiplexing (OFDM) based transmission. We employ a hybrid precoding (HP) architecture with three stages: (i) radio frequency (RF) beamformer, (ii) baseband (BB) precoder, (iii) resource allocation (RA) block. First, a single RF beamformer block is built for all subcarriers via the slow time-varying angle-of-departure (AoD) information. For enhancing the energy efficiency, the RF beamformer aims to reduce the hardware cost/complexity and total power consumption via a low number of RF chains. Afterwards, the reduced-size effective channel state information (CSI) is utilized in the design of a distinct BB precoder and RA block for each subcarrier. The BB precoder is developed via regularized zero-forcing technique. Finally, the RA block is built via the proposed GA-RA technique for throughput maximization by allocating the power and subcarrier resources. The illustrative results show that the throughput performance in the MU-mMIMO-OFDM systems is greatly enhanced via the proposed GA-RA technique compared to both equal RA (EQ-RA) and particle swarm optimization based RA (PSO-RA). Moreover, the performance gain ratio increases with the increasing number of subcarriers, particularly for low transmission powers.