The application of Machine Learning (ML) techniques to complex engineering problems has proved to be an attractive and efficient solution. ML has been successfully applied to several practical tasks like image recognition, automating industrial operations, etc. The promise of ML techniques in solving non-linear problems influenced this work which aims to apply known ML techniques and develop new ones for wireless spectrum sharing between Wi-Fi and LTE in the unlicensed spectrum. In this work, we focus on the LTE-Unlicensed (LTE-U) specification developed by the LTE-U Forum, which uses the duty-cycle approach for fair coexistence. The specification suggests reducing the duty cycle at the LTE-U base-station (BS) when the number of co-channel Wi-Fi basic service sets (BSSs) increases from one to two or more. However, without decoding the Wi-Fi packets, detecting the number of Wi-Fi BSSs operating on the channel in real-time is a challenging problem. In this work, we demonstrate a novel ML-based approach which solves this problem by using energy values observed during the LTE-U OFF duration. It is relatively straightforward to observe only the energy values during the LTE-U BS OFF time compared to decoding the entire Wi-Fi packet, which would require a full Wi-Fi receiver at the LTE-U base-station. We implement and validate the proposed ML-based approach by real-time experiments and demonstrate that there exist distinct patterns between the energy distributions between one and many Wi-Fi AP transmissions. The proposed ML-based approach results in a higher accuracy (close to 99\% in all cases) as compared to the existing auto-correlation (AC) and energy detection (ED) approaches.