Accurate estimation of the distance between the transmitter (TX) and the receiver (RX) in molecular communication (MC) systems can provide faster and more reliable communication. Existing theoretical models in the literature are not suitable for distance estimation in a practical scenario. Furthermore, deriving an analytical model is not easy due to effects such as boundary conditions in the diffusion process, the initial velocity of the molecules and unsteady flows. Therefore, five different practical methods comprising three novel data analysis based methods and two supervised machine learning (ML) methods, Multivariate Linear Regression (MLR) and Neural Network Regression (NNR), are proposed for distance estimation at the RX side. In order to apply the ML methods, a macroscale practical MC system, which consists of an electric sprayer without a fan, alcohol molecules, an alcohol sensor and a microcontroller, is established, and the received signals are recorded. A feature extraction algorithm is proposed to utilize the measured signals as the inputs in ML methods. The numerical results show that the ML methods outperform the data analysis based methods in the root mean square error sense with the cost of complexity. Moreover, the peak time based estimation, which is one of the proposed data analysis based methods, yields better results with respect to the other proposed four methods, as the distance increases. Given the experimental data and fluid dynamics theory, a possible trajectory of the molecules between the TX and RX is given. Our findings show that distance estimation performance is jointly affected by unsteady flows and the non-linearity of the sensor. According to our findings based on fluid dynamics, it is evaluated that fluid dynamics should be taken into account for more accurate parameter estimation in practical macroscale MC systems.