Despite the development of two and three dimensional (2D&3D) technology, it has attracted the attention of researchers in recent years. This research is done to reveal the detailed effects of 2D in comparison with 3D technology on the human brain waves. The impact of 2D&3D video watching using electroencephalography (EEG) brain signals is studied. A group of eight healthy volunteers with the average age of 31+-3.06 years old participated in this three-stage test. EEG signal recording consisted of three stages: After a bit of relaxation (a), a 2D video was displayed (b), the recording of the signal continued for a short period of time as rest (c), and finally the trial ended. Exactly the same steps were repeated for the 3D video. Power spectrum density (PSD) based on short time Fourier transform (STFT) was used to analyze the brain signals of 2D&3D video viewers. After testing all the EEG frequency bands, delta and theta were extracted as the features. Partial least squares regression (PLSR) and Support vector machine (SVM) classification algorithms were considered in order to classify EEG signals obtained as the result of 2D&3D video watching. Successful classification results were obtained by selecting the correct combinations of effective channels representing the brain regions.