In this paper, we investigate the sensing-aided physical layer security (PLS) towards Integrated Sensing and Communication (ISAC) systems. A well-known limitation of PLS is the need to have information about potential eavesdroppers (Eves). The sensing functionality of ISAC offers an enabling role here, by estimating the directions of potential Eves to inform PLS. In our approach, the ISAC base station (BS) firstly emits an omni-directional waveform to search for potential Eves' directions by employing the combined Capon and approximate maximum likelihood (CAML) technique. Using the resulting information about potential Eves, we formulate secrecy rate expressions, that are a function of the Eves' estimation accuracy. We then formulate a weighted optimization problem to simultaneously maximize the secrecy rate and minimize the CRB with the aid of the artificial noise (AN), and minimize the CRB of targets'/Eves' estimation. By taking the possible estimation errors into account, we enforce a beampattern constraint with a wide main beam covering all possible directions of Eves. This implicates that security needs to be enforced in all these directions. By improving estimation accuracy, the sensing and security functionalities provide mutual benefits, resulting in improvement of the mutual performances with every iteration of the optimization, until convergence. Our results avail of these mutual benefits and reveal the usefulness of sensing as an enabler for practical PLS.