Background: Several studies have shown that both respiratory and cardiac motion can be extracted from the Pilot Tone (PT) signal successfully. However, most of these studies were performed in healthy volunteers. In addition, validating PT using ECG as a reference can be problematic because both PT and ECG tend to be unreliable in patients with arrhythmias. Purpose: We seek to evaluate the accuracy and reliability of the cardiac and respiratory signals extracted from PT in patients clinically referred for cardiovascular MRI with the image-derived signals as the reference. Methods: Twenty-three patients were scanned on a 1.5 T scanner using balanced steady-state free-precession real-time (RT) cine sequence. The PT signal was generated by a built-in PT transmitter integrated within the body array coil. For comparison, commercial ECG and BioMatrix (BM) respiratory sensor signals were synchronously recorded. Results: The respiratory motion extracted from PT correlated positively with the image-derived respiratory signal in all cases and showed a stronger correlation (absolute coefficient: 0.95+-0.09) than BM (0.72+-0.24). For the cardiac signal, PT trigger jitter (standard deviation of PT trigger locations relative to ECG triggers) ranged from 6.6 to 83.3 ms, with a median of 21.8 ms. The mean absolute difference between the PT and corresponding ECG cardiac cycle duration was less than 5% of the averaged ECG RR interval for 21 out of 23 patients. Overall, the performance of PT-based trigger extraction was comparable to that of ECG. We did not observe significant linear dependence (p>0.28) of PT delay and PT jitter on the patients' BMI or cardiac cycle duration. Conclusions: This study demonstrates the potential of PT to monitor both respiratory and cardiac motion in patients clinically referred for cardiovascular MRI.