The vehicular-to-everything (V2X) technology has recently drawn a number of attentions from both academic and industrial areas. However, the openness of the wireless communication system makes it more vulnerable to identity impersonation and information tampering. How to employ the powerful radio frequency fingerprint (RFF) identification technology in V2X systems turns out to be a vital and also challenging task. In this paper, we propose a novel RFF extraction method for Long Term Evolution-V2X (LTE-V2X) systems. In order to conquer the difficulty of extracting transmitter RFF in the presence of wireless channel and receiver noise, we first estimate the wireless channel which excludes the RFF. Then, we remove the impact of the wireless channel based on the channel estimate and obtain initial RFF features. Finally, we conduct RFF denoising to enhance the quality of the initial RFF. Simulation and experiment results both demonstrate that our proposed RFF extraction scheme achieves a high identification accuracy. Furthermore, the performance is also robust to the vehicle speed.