Voice-driven services (VDS) are being used in a variety of applications ranging from smart home control to payments using digital assistants. The input to such services is often captured via an open voice channel, e.g., using a microphone, in an unsupervised setting. One of the key operational security requirements in such setting is the freshness of the input speech. We present AEOLUS, a security overlay that proactively embeds a dynamic acoustic nonce at the time of user interaction, and detects the presence of the embedded nonce in the recorded speech to ensure freshness. We demonstrate that acoustic nonce can (i) be reliably embedded and retrieved, and (ii) be non-disruptive (and even imperceptible) to a VDS user. Optimal parameters (acoustic nonce's operating frequency, amplitude, and bitrate) are determined for (i) and (ii) from a practical perspective. Experimental results show that AEOLUS yields 0.5% FRR at 0% FAR for speech re-use prevention upto a distance of 4 meters in three real-world environments with different background noise levels. We also conduct a user study with 120 participants, which shows that the acoustic nonce does not degrade overall user experience for 94.16% of speech samples, on average, in these environments. AEOLUS can therefore be used in practice to prevent speech re-use and ensure the freshness of speech input.