The standard theory of optimal stopping is based on the idealised assumption that the underlying process is essentially known. In this paper, we drop this restriction and study data-driven optimal stopping for a general diffusion process, focusing on investigating the statistical performance of the proposed estimator of the optimal stopping barrier. More specifically, we derive non-asymptotic upper bounds on the simple regret, along with uniform and non-asymptotic PAC bounds. Minimax optimality is verified by completing the upper bound results with matching lower bounds on the simple regret. All results are shown both under general conditions on the payoff functions and under more refined assumptions that mimic the margin condition used in binary classification, leading to an improved rate of convergence. Additionally, we investigate how our results on the simple regret transfer to the cumulative regret for a specific exploration-exploitation strategy, both with respect to lower bounds and upper bounds.