The Markov chain approximation of a one-dimensional symmetric diffusion is investigated in this paper. Given an irreducible reflecting diffusion on a closed interval with scale function $s$ and speed measure $m$, the approximating Markov chains are constructed explicitly through the trace of the Dirichlet form corresponding to the diffusion. One feature of our approach is that it does not require uniform ellipticity on diffusion coefficient of the limit object or uniform regularity on conductances of the approximative Markov chains, as imposed usually in the previous related works.