We prove the sharp Gaussian isoperimetric inequality for conjugate heat-kernel measures along a Ricci flow via a monotonicity formula. As consequences, we obtain the exact Gaussian enlargement theorem and a Gaussian-quantile two-set concentration estimate. In particular, this recovers the exponential concentration estimate of Hein--Naber from a sharper isoperimetric profile. We also derive Gaussian rearrangement inequalities, recover the sharp Hein--Naber log-Sobolev inequality, and identify the universal Gaussian-model constants in Bamler's \(L^p\)-Poincaré inequalities. Further applications include Gaussian-profile localization near Bamler's \(H_n\)-centers, convex-order and moment estimates for logarithmic derivatives of the conjugate heat kernel, reverse hypercontractivity, entropy-regular profile stability, and a path-space Bobkov inequality.