In the theory of species, differential as well as integral operators are known to arise in a natural way. In this paper, we shall prove that they precisely fit together in the algebraic framework of integro-differential rings, which are themselves an abstraction of classical calculus (incorporating its Fundamental Theorem). The results comprise (set) species as well as linear species. Localization of (set) species leads to the more general structure of modified integro-differential rings, previously employed in the algebraic treatment of Volterra integral equations. Furthermore, the ring homomorphism from species to power series via taking generating series is shown to be a (modified) integro-differential ring homomorphism. As an application, a topology and further algebraic operations are imported to virtual species from the general theory of integro-differential rings.