We prove that checking if a partial matrix is partial totally positive is co-NP-complete. This contrasts with checking a conventional matrix for total positivity, for which we provide a cubic time algorithm. Checking partial sign regularity with any signature, including partial total nonnegativity, is also co-NP-complete. Finally, we prove that checking partial total positivity in a partial matrix with logarithmically many unspecified entries may be done in polynomial time.