The communication matrix for two-way deterministic finite automata (2DFA) with $n$ states is defined for an automaton over a full alphabet of all $(2n+1)^n$ possible symbols: its rows and columns are indexed by strings, and the entry $(u, v)$ is $1$ if $uv$ is accepted by the automaton, and $0$ otherwise. With duplicate rows and columns removed, this is a square matrix of order $n(n^n-(n-1)^n)+1$, and its rank is known to be a lower bound on the number of states necessary to transform an $n$-state 2DFA to a one-way unambiguous finite automaton (UFA). This paper determines this rank, showing that it is exactly $f(n)=\sum_{k=1}^n \binom{n}{k-1} \binom{n}{k} \binom{2k-2}{k-1} =(1+o(1)) \frac{3\sqrt{3}}{8πn} 9^n$, and this function becomes the new lower bound on the state complexity of the 2DFA to UFA transformation, thus improving a recent lower bound by S. Petrov and Okhotin (``On the transformation of two-way deterministic finite automata to unambiguous finite automata'', Inf. Comput., 2023). The key element of the proof is determining the rank of a $k! \times k!$ submatrix, with its rows and columns indexed by permutations, where the entry $(π, σ)$ is $1$ if $σ\circ π$ is a cycle of length $k$, and 0 otherwise; using the methods of group representation theory it is shown that its rank is exactly $\binom{2k-2}{k-1}$, and this implies the above formula for $f(n)$.