We construct the twist automorphism of open Richardson varieties inside the flag variety of a complex semisimple algebraic group. We show that the twist map preserves totally positive parts, and prove a Chamber Ansatz formula for it. Our twist map generalizes the twist maps previously constructed by Berenstein-Fomin-Zelevinsky, Marsh-Scott, and Muller-Speyer. We use it to explain the relationship between the two conjectural cluster structures for Richardson varieties studied by Leclerc and by Ingermanson.