For a nonnegative integer $k$, a graph $G$ is said to be $k$-factor-critical if $G-Q$ admits a perfect matching for any $Q\subseteq V(G)$ with $|Q|=k$. In this article, we prove spectral radius conditions for the existence of $k$-factor-critical graphs. Our result generalises one previous result on perfect matchings of graphs. Furthermore, we claim that the bounds on spectral radius in Theorem 3.1 are sharp.