For a connected graph $G$, we present the concept of a new graph matrix related to its distance and Seidel matrix, called distance Seidel matrix $\mathcal{D}^S(G)$. Suppose that the eigenvalues of $\mathcal{D}^S(G)$ be $\partial_{1}^{S}(G) \geq \cdots \geq \partial_{n}^{S}(G).$ In this article, we establish a relationship between distance Seidel eigenvalues of a graph with its distance and adjacency eigenvalues. We characterize all the connected graphs with $\partial_{1}^{S}(G)= 3.$ Also, we determine different bounds for the distance Seidel spectral radius and distance Seidel energy. We study the distance Seidel energy change of the complete bipartite graph due to the deletion of an edge. Moreover, we obtain the distance Seidel spectra of different graph operations such as join, cartesian product, lexicographic product, and unary operations like the double graph and extended double cover graph. We give various families of distance Seidel cospectral and distance Seidel integral graphs as an application.