Let $G$ be a graph and $T$ a certain connected subgraph of $G$. The $T$-structure connectivity $κ(G; T)$ (or resp., $T$-substructure connectivity $κ^{s}(G; T)$) of $G$ is the minimum number of a set of subgraphs $\mathcal{F}=\{T_{1}, T_{2}, \ldots, T_{m}\}$ (or resp., $\mathcal{F}=\{T^{'}_{1}, T^{'}_{2}, \ldots, T^{'}_{m}\}$) such that $T_{i}$ is isomorphic to $T$ (or resp., $T^{'}_{i}$ is a connected subgraph of $T$) for every $1\leq i \leq m$, and $\mathcal{F}$'s removal will disconnect $G$. The twisted hypercube $H_{n}$ is a new variant of hypercubes with asymptotically optimal diameter introduced by X.D. Zhu. In this paper, we will determine both $κ(H_{n}; T)$ and $κ^{s}(H_{n}; T)$ for $T\in\{K_{1,r}, P_{k}\}$, respectively, where $3\leq r\leq 4$ and $1 \leq k \leq n$.