The well-known characterization of two-ended groups says that every two-ended group can be split over finite subgroups which means it is isomorphic to either by a free product with amalgamation $A\ast_C B$ or an HNN-extension $\ast_φ C$, where $C$ is a finite group and $[A:C]=[B:C]=2$ and $φ\in Aut(C)$. In this paper, we show that there is a way in order to spilt two-ended quasi-transitive graphs without dominated ends and two-ended transitive graphs over finite subgraphs in the above sense. As an application of it, we characterize all groups acting with finitely many orbits almost freely on those graphs.