An edge-colouring of a graph is distinguishing, if the only automorphism which preserves the colouring is the identity. It has been conjectured that all but finitely many connected, finite, regular graphs admit a distinguishing edge-colouring with two colours. We show that all such graphs except $K_2$ admit a distinguishing edge-colouring with three colours. This result also extends to infinite, locally finite graphs. Furthermore, we are able to show that there are arbitrary large infinite cardinals $κ$ such that every connected $κ$-regular graph has distinguishing edge-colouring with two colours.