Let $n$, $k$ and $l$ be integers with $1\leq k<l\leq n-1$. The set-inclusion graph $G(n,k,l)$ is the graph whose vertex set consists of all $k$- and $l$-subsets of $[n]=\{1,2,\ldots,n\}$, where two distinct vertices are adjacent if one of them is contained in another. In this paper, we determine the spectrum and automorphism group of $G(n,k,l)$, respectively.