For a simple signed graph $G$ with the adjacency matrix $A$ and net degree matrix $D^{\pm}$, the net Laplacian matrix is $L^{\pm}=D^{\pm}-A$. We introduce a new oriented incidence matrix $N^{\pm}$ which can keep track of the sign as well as the orientation of each edge of $G$. Also $L^{\pm}=N^{\pm}(N^{\pm})^T$. Using this decomposition, we find the numbers of positive and negative spanning trees of $G$ in terms of the principal minors of $L^{\pm}$ generalizing Matrix Tree Theorem for an unsigned graph. We present similar results for the signless net Laplacian matrix $Q^{\pm}=D^{\pm}+A$ along with a combinatorial formula for its determinant.