In the literature, several identification problems in graphs have been studied, of which, the most widely studied are the ones based on dominating sets as a tool of identification. Hereby, the objective is to separate any two vertices of a graph by their unique neighborhoods in a suitably chosen dominating or total-dominating set. Such a (total-)dominating set endowed with a separation property is often referred to as a code of the graph. In this paper, we study the four separation properties location, closed-separation, open-separation and full-separation. We address the complexity of finding minimum separating sets in a graph and study the interplay of these separation properties with several codes (establishing a particularly close relation between separation and codes based on domination) as well as the interplay of separation and complementation (showing that location and full-separation are the same on a graph and its complement, whereas closed-separation in a graph corresponds to open-separation in its complement).