A non-commuting graph of a finite group $G$ is a graph whose vertices are non-central elements of $G$ and two vertices are adjacent if they don't commute in $G$. In this paper, we study the non-commuting graph of the group $U_{6n}$ and explore some of its properties including the independent number, clique and chromatic numbers. Also, the general formula of the resolving polynomial of the non-commuting graph of the group $U_{6n}$ are provided. Furthermore, we find the detour index, eccentric connectivity, total eccentricity and independent polynomials of the graph.