In this paper we study a relation between the cohomology ring of a regular nilpotent Hessenberg variety and Schubert polynomials. To describe an explicit presentation of the cohomology ring of a regular nilpotent Hessenberg variety, polynomials $f_{i,j}$ were introduced by Abe-Harada-Horiguchi-Masuda. We show that every polynomial $f_{i,j}$ is an alternating sum of certain Schubert polynomials.